DATASHEET
Formulation
Solid
Storage:
Stable for 2 years after receipt when stored at -20°C.
Product Information:
CAS Number: 35340-49-9
Molecular formula: C24H18O7
Molecular weight: 418.4
Purity: >97% (NMR)
Appearance: Solid.
Solubility: Soluble in chloroform.
InChiKey: YKSJJXGQHSESKB-UHFFFAOYSA-N
SMILES: CC(=O)OC1=CC2=C(C=C1)C(C1=C(O2)C=C(OC(C)=O)C=C1)C1=C(C=CC=C1)C(O)=O
SMILES: CC(=O)OC1=CC2=C(C=C1)C(C1=CC=CC=C1C(O)=O)C1=C(O2)C=C(OC(C)=O)C=C1
Description:
Dihydrofluorescein diacetate is a fluorescent probe for detecting intracellular oxidants. It is hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as Dihydrorhodamine 123 (Ex/Em: 490/514nm).