DATASHEET
Formulation
Powder
Storage:
Stable for 2 years after receipt when stored at -20°C.
Product Information:
CAS Number: 126769-01-5
Molecular formula: C15H9NO3
Molecular weight: 251.24
Purity: >95% (HPLC)
Appearance: White to off-white powder.
Solubility: Soluble in methanol.
InChiKey: PNCHURHVMDRFTR-UHFFFAOYSA-N
SMILES: [H]C(=O)C1=NC2=CC=CC=C2C=C1C(=O)C1=CC=CO1
SMILES: O=CC1=NC2=CC=CC=C2C=C1C(=O)C1=CC=CO1
Description:
3-(2-Furoyl)quinoline-2-carboxaldehyde (FQCA) is a fluorogenic derivatizing reagent for liquid chromatography suitable for high-sensitivity analysis of amino acids by laser-induced fluorescence with an argon-ion laser. The reagent forms highly fluorescent isoindoles upon reaction with primary amines. It is used for the determination of isoelectronic points (pI values) of proteins with laser-induced fluorescence detection (c-IEF-LIF). C-IEF-LIF technique can routinely detect 10-11 M of FQCA-labeled protein, compared with 10-7 M by traditional slab gel IEF and silver staining. Spectral Data: lambdaex=~480nm, lambdaem=~590nm.