DATASHEET
Formulation
Solid
Storage:
Stable for 2 years after receipt when stored at -20°C.
Product Information:
CAS Number: 75350-46-8
Molecular formula: C24H13NO7
Molecular weight: 427.36
Purity: >90% (HPLC)
Appearance: Orange to brown solid.
Solubility: Soluble in DMF.
InChiKey: AYDAHOIUHVUJHQ-UHFFFAOYSA-N
SMILES: OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N1C(=O)C=CC1=O)C1=C(O2)C=C(O)C=C1
SMILES: OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N1C(=O)C=CC1=O)C1=C(O2)C=C(O)C=C1
Description:
Green fluorescent thiol-reactive dye (Ex/Em: 494/519nm) for bioconjugation, widely used as protein and peptide fluorescent labeling reagent, easily conjugating fluorescein. The reaction requires very mild condition and is highly specific. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.