bs-76409C

DATASHEET

Formulation Solid

Storage: Stable for 2 years after receipt when stored at +4°C.

Product Information:

CAS Number: 223769-64-0

Molecular formula: C16H16N4O . 2CH4O3S

Molecular weight: 280.3 . 192.2

Purity: >95% (HPLC)

Appearance: Yellow solid.

Solubility: Soluble in water or DMSO (20mg/ml).

InChiKey: YGNSQKCULHSJDC-HFPMQDOPSA-N

SMILES: OC1=C(/C=C/C2=CC=C(C([NH3+])=N)C=C2)C=CC(C([NH3+])=N)=C1.CS(=O)([O-])=O.CS(=O)([O-])=O

SMILES: CS(O)(=O)=O.CS(O)(=O)=O.NC(=N)C1=CC=C(C=CC2=CC=C(C=C2O)C(N)=N)C=C1

Description:

Hydroxystilbamidine (also called Fluoro Gold) is a cationic dye used for staining DNA and RNA. It exhibits distinctively different fluorescence emission profiles when bound to DNA compared to RNA. It is also frequently used as a retrograde neuronal tracer. The fluorescent tracer provides intense retrograde labeling that is extremely sensitive and reliable, does not diffuse out of retrograde-labeled neurons, and can be pressure-injected or introduced by iontophoresis into cells. Hydroxystilbamidine is compatible with most frequently used neuroanatomical techniques such as immunofluorescence, immunocytochemistry, autoradiography, and horseradish peroxidase-based histochemistry, paraffin embedding. Gold color is emitted when tissue is processed with a neutral pH buffer while blue/bright white is emitted when an acidic pH buffer is used. Spectral data: lambdaex=361nm and lambdaem=536nm. In addition, hydroxystilbamidine is an amidine antibiotic with antibacterial antifungal, antitrypanosomal, antimalarial and carcinostatic activities. Binds to DNA and RNA in a non-intercalating manner, and is a powerful inhibitor of ribonucleases. Binds also to lysosomes.