bs-76446C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 161617-45-4

Molecular formula: C19H10IN6NaO10S2

Molecular weight: 696.34

Purity: >90% (HPLC)

Appearance: Pale yellow powder.

Solubility: Soluble in chloroform.

InChiKey: LKVNWZJLLRMMAS-UHFFFAOYSA-M

SMILES: [Na+].[O-][N+](=O)C1=CC=C(N2N=C(N=[N+]2C2=CC=C(I)C=C2)C2=CC=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C(=C1)[N+]([O-])=O

Description:

4-[3-(4-iodophenyl)-2-(2,4-dinitrophenyl)-2H-5-tetrazolio]-1,3-benzenedisulfonate sodium salt (IPB) have found application in clinical assays and cell proliferation assays that are based on a spectrophotometric measurement of dehydrogenase activities of cells. Disulfonated tetrazolium salt IPB, that produces a highly water-soluble formazan dye that proved to be extremely useful as a cell-viability indicator. IPB is reduced by reduced nicotinamide adenine dinucleotide (NADH) to formazan in the presence of a redox mediator such as 1-methoxy PMS, which provides the basis to specrophotometric assays of NADH.