DATASHEET
Formulation
Solid
Storage:
Stable for 2 years after receipt when stored at -20°C.
Product Information:
CAS Number: 251292-24-7
Molecular formula: C17H15NO5
Molecular weight: 313.3
Purity: >97% (HPLC)
Appearance: Solid.
Solubility: Soluble in DMSO.
InChiKey: RVDFSVVDUVHHKE-UHFFFAOYSA-N
SMILES: CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2
SMILES: CC(C)COC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2
Description:
Cell permeable resorufin derivative used as a fluorogenic indicator for cell viability. Resorufin was selected as the fluorophore because it fluoresces at a longer wavelength and is amenable to modification at the hydroxyl group which is essential for its fluorescence. Incubation with esterase at pH 8.0 results in a 80-90 nm shift of emission max. (Ex/Em: 500/~593nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)). This cell viability indicator is 2 times more sensitive than calcein-AM.