bs-76498C [Life Science]
4-Methylumbelliferyl phosphate disodium salt
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Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 22919-26-2

Molecular formula: C10H7Na2O6P

Molecular weight: 300.11

Purity: >97% (NMR)

Appearance: White to off-white powder.

Solubility: Soluble in water (50 mg/ml).

InChiKey: WUUDJQVNZPEPKN-UHFFFAOYSA-L

SMILES: CC(C1=CC=C(OP([O-])([O-])=O)C=C1O2)=CC2=O.[Na+].[Na+]

SMILES: [Na+].[Na+].CC1=CC(=O)OC2=CC(OP([O-])([O-])=O)=CC=C12

Description:

4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate for phosphatases (mainly alkaline). It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. Therefore, this phosphatase substrate is not well suited for living cell or continuous assays since 4-MU only develops maximum fluorescence at a pH value of >10. Spectral data: lambdaex=319nm; lambdaem=384nm (pH 9.0). lambdaex=360nm; lambdaem=449nm (for reaction product).

Size: 250mg, 1g

Applications: Life Science

For research use only. Not intended for diagnostic or therapeutic use.