bs-76582C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 139332-66-4

Molecular formula: C10H11N5O3

Molecular weight: 249.23

Purity: ≥98% (NMR)

Appearance: Red to brown powder.

Solubility: Soluble in DMSO.

InChiKey: MVLWYDGJBGPXOL-UHFFFAOYSA-N

SMILES: [O-][N+](=O)C1=CC=C(N2CCNCC2)C2=NON=C12

SMILES: [O-][N+](=O)C1=CC=C(N2CCNCC2)C2=NON=C12

Description:

This common fluorescent reagent often used in carbohydrate analysis, is also useful in fluorescence visualization of lysosomes in live cell lines in culture. This fluorophore that has the unusual environmentally sensitive property of increasing fluorescence in low pH environments. Comparison of staining patterns with the common LysoTracker™ or LysoSensor™ dyes indicated comparable staining, albeit using a higher concentration of NBD-piperazine (1 µM versus 100 nM for the MP dyes) but at a lower cost per assay. Also used for the determination of mono- and diisocyantes in air samples, and determination of lactate, hydroxybutyrate and other intermediates in body fluids. The piperazino moiety reacts readily with isocyantes to form the corresponding urea-derivatives. The derivatives can be detected by LC with UV or fluorescent-detection. Spectral data: lambdaex=470nm, lambdaem=540nm.