Host: Rabbit
Target Protein: GPR65
Immunogen Range: 72-89/337
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8477
Swiss Prot: Q8IYL9
Source: KLH conjugated synthetic peptide derived from human GPR65
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 39
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Bortell et al. Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro. (2017) J.Neuroinflammatio. 14:49Read more>>
- Daichi Mori. et al. Extracellular acidity in tumor tissue upregulates programmed cell death protein?1 expression on tumor cells via proton-sensing G protein-coupled receptors. 2021 Sep 13Read more>>
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR65 Polyclonal Antibody, Unconjugated (bs-7668R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates probed with GPR65 Polyclonal Antibody, Unconjugated (bs-7668R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR65 Polyclonal Antibody, Unconjugated (bs-7668R) at 1:50 overnight at 4°C, followed by a conjugated secondary antibody and DAPI for nuclei staining.