bs-76737C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 124549-11-7

Molecular formula: C46H46N2O16

Molecular weight: 882.86

Purity: ~80% (HPLC)

Appearance: Brownish-yellow powder.

Solubility: Soluble in methanol or DMSO.

InChiKey: YOQMJMHTHWYNIO-UHFFFAOYSA-N

SMILES: COC1=CC2=C(OC(C3=C(C(O)=O)C=C(C(O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(O)=O)C=C(C(O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4

SMILES: COC1=C(C=C2OC(=CC2=C1)C1=C(C=C(C=C1)C(O)=O)C(O)=O)N1CCOCCOCCN(CCOCCOCC1)C1=C(OC)C=C2C=C(OC2=C1)C1=C(C=C(C=C1)C(O)=O)C(O)=O

Description:

PBFI is a cell-impermeant fluorescent indicator for potassium ions, suitable for detection of physiological concentrations of K+ in the presence of other monovalent cations. PBFI is used to measure intracellular potassium (K+) fluxes in animal cells and in plant cells and vacuoles. The observation that intracellular K+ levels are a controlling factor in apoptotic cell death pathways, increased the interest for PBFI. PBFI has been used for detecting adrenoceptor-stimulated decreases of intracellular K+ concentration in astrocytes and neurons, evaluating the mediating effects of K+ depletion on monocytic cell necrosis, measuring intracellular K+ fluxes associated with apoptotic cell shrinkage, monitoring mitochondrial KATP channel activation, or detecting elevated intracellular K+ levels associated with HIV-induced cytopathology. Flow cytometric measurements using UV argon-ion laser excitation (351nm and 364nm) of PBFI indicate that K+ efflux induces shrinkage of apoptotic cells and is a trigger for caspase activation. Spectral data: lambdaex 336nm;, lambdaem 557nm.