bs-76749C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 87687-02-3

Molecular formula: C19H13NO3

Molecular weight: 303.31

Purity: >98% (HPLC)

Appearance: Orange to red powder.

Solubility: Soluble in DMSO.

InChiKey: XNZRYTITWLGTJS-UHFFFAOYSA-N

SMILES: O=C1C=C2OC3=CC(OCC4=CC=CC=C4)=CC=C3N=C2C=C1

SMILES: O=C1C=CC2=NC3=C(OC2=C1)C=C(OCC1=CC=CC=C1)C=C3

Description:

Resorufin benzyl ether (7-Benzyloxyresorufin) is a fluorogenic substrate widely used for monitoring cytochrome P450 (CYP450) activities in cell extracts and solutions. It is the model substrate for 7-Benzoxyresorufin-O-dealkylase (BROD) assays. It is typically used near its apparent Km value of 30 �M to screen the inhibition/activation potential of test compounds, to predict potential drug-drug interactions, or to monitor other CYP450 activities. Cytochrome P450 oxidase is a large number of evolutionary related oxidative enzymes important in animal, plant, and bacterial physiology. Most cytochromes P450 (CYPs) have about 500 amino acids and a heme group at the active site. Upon enzymatic cleavage of 7-Benzyloxyresorufin by CYP450, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=585nm.