bs-76753C

DATASHEET

Formulation Powder

Storage: Stable for 2 years after receipt when stored at -20°C.

Product Information:

CAS Number: 1152-14-3

Molecular formula: C14H9NO4

Molecular weight: 255.23

Purity: >98% (TLC)

Appearance: Orange to dark orange powder.

Solubility: Soluble in DMSO (5mg/ml) or DMF (20mg/ml).

InChiKey: UJWKHDKBOVPINX-UHFFFAOYSA-N

SMILES: O=C1C=C2OC3=CC(OC(C)=O)=CC=C3N=C2C=C1

SMILES: CC(=O)OC1=CC2=C(C=C1)N=C1C=CC(=O)C=C1O2

Description:

Resorufin acetate is a fluorogenic substrate for hydrolytic esterases and cellulases used for monitoring cytosolic aldehyde dehydrogenase (ALDH1A1) esterase activity, chymotrypsin activity. Resorufin acetate has been used as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase (GOD), without significant effects of ascorbic acid, uric acid, or bilirubin. It also has been used for the chromogenic and fluorescent detection of hydrazine, acyl protein thioesterase (APT) enzymes and perborate ions, and reveals a selective turn-on type chromogenic and fluorogenic signaling behavior based on the selective and efficient cleavage of acetate group. Upon enzymatic cleavage of the acetate group of resorufin acetate, the fluorescent hydrolysis product resorufin is released and its fluorescence can be used to quantify enzyme activity. Spectral data of Resorufin: lambdaex=571nm, lambdaem=585nm.