Host: Rabbit
Target Protein: TP53TG5
Immunogen Range: 21-120/290
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 27296
Source: KLH conjugated synthetic peptide derived from human TP53TG5
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 34
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Dog
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: Siha cell lysates probed with TP53TG5 Polyclonal Antibody, Unconjugated (bs-7702R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TP53TG5 Polyclonal Antibody, Unconjugated (bs-7702R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TP53TG5 Polyclonal Antibody, Unconjugated (bs-7702R) at 1:200 overnight at 4°C, DAB staining.