bs-7913R-A647 [Conjugated Primary Antibody]
LATS1 (Thr1079) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LATS1 Thr1079

Specificity: This sequence will cross react with LATS2 if phosphorylated at Thr1041

Modification Site: Thr1079

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9113

Swiss Prot: O95835

Source: KLH conjugated synthetic phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 124


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Grijalva, James, et al. "Dynamic Alterations in Hippo Signaling Pathway and YAP Activation during Liver Regeneration." American Journal of Physiology-Gastrointestinal and Liver Physiology (2014).Read more>>
  • Konishi,et al.Proliferation of hepatic stellate cells, mediated by YAP and TAZ, contributes to liver repair and regeneration after liver ischemia-reperfusion injury.(2018) American Journal of Physiology. Gastrointestinal and Liver Physiology. 314:G471-G482.Read more>>
  • Fatah,et al.Reduced Proximal Tubular Expression of Megalin and Neonatal Fc Receptor in Proteinuria: The Role of Matrix Metalloproteinases.(2018) . :.Read more>>
  • Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617. Read more>>
  • Wang C et al. MicroRNA\10a promotes epithelial\to\mesenchymal transition and stemness maintenance of pancreatic cancer stem cells via upregulating the Hippo signaling pathway through WWC2 inhibition. J Cell Biochem. 2020 Nov;121(11):4505-4521.Read more>>
  • Lixia Chen. et al. CSRP2 suppresses colorectal cancer progression via p130Cas/Rac1 axis-meditated ERK, PAK, and HIPPO signaling pathways.. Theranostics. 2020; 10(24): 11063C11079Read more>>
  • Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254Read more>>
  • Akiko Sato. et al. Pharmacological Activation of YAP/TAZ by Targeting LATS1/2 Enhances Periodontal Tissue Regeneration in a Murine Model. INT J MOL SCI. 2023 Jan;24(2):97Read more>>
  • Pin-Shi Ni. et al. Indirect regulation of HIPPO pathway by miRNA mediates high-intensity intermittent exercise to ameliorate aging skeletal muscle function. SCAND J MED SCI SPOR. 2023 FebRead more>>
  • Li Mengzhi. et al. NRP1 transduces mechanical stress inhibition via LATS1/YAP in hypertrophic scars. CELL DEATH DISCOV. 2023 Sep;9(1):1-1Read more>>
VALIDATION IMAGES