DATASHEET
Host:
Rabbit
Target Protein:
Ubiquitin
Immunogen Range:
63-74/76
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
7314
Swiss Prot:
P0CG48
Source:
KLH conjugated synthetic peptide derived from human Ubiquitin C-terminuse
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Conjugation:
Biotin
Excitation/ Emission:
N/A
Size:
100µL
Concentration:
1ug/ul
Applications:
WB(1:500-1000)
ELISA(1:200-400)
IHC-P(1:100-500)
IHC-F(1:50-200)
Human
Predicted Cross Reactive Species:
Mouse
Cow
Sheep
Chicken
For research use only. Not intended for diagnostic or therapeutic use.