DATASHEET
Host:
Rabbit
Target Protein:
AKR1A1
Immunogen Range:
53-130/325
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
10327
Swiss Prot:
P14550
Source:
KLH conjugated synthetic peptide derived from human AKR1A1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).
VALIDATION IMAGES
Lane 1: mouse pancreas lysates Lane 2: mouse brain lysates probed with Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-1803R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 36kD. Observed band size: 36kD.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver cell lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.