bs-7953R [Primary Antibody]
AKR1A1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AKR1A1

Immunogen Range: 53-130/325


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10327

Swiss Prot: P14550

Source: KLH conjugated synthetic peptide derived from human AKR1A1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 36


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: mouse pancreas lysates Lane 2: mouse brain lysates probed with Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-1803R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 36kD. Observed band size: 36kD.


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse liver lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Liver cell lysates probed with AKR1A1 Polyclonal Antibody, Unconjugated (bs-7953R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.