bs-7969R [Primary Antibody]
ARMC3 (Cancer/testis antigen 81) Polyclonal Antibody
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Host: Rabbit

Target Protein: ARMC3 (Cancer/testis antigen 81)

Immunogen Range: 752-840/872


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 219681

Swiss Prot: Q5W041

Source: KLH conjugated synthetic peptide derived from human ARMC3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

ARMC3 contains 12 ARM repeats. ARM (Armadillo/beta-catenin-like) domains are imperfect 45-amino acid repeats involved in protein-protein interactions. ARM domain-containing proteins, such as ARMC3, function in signal transduction, development, cell adhesion and mobility, and tumor initiation and metastasis. The specific function of ARMC3 is not yet known.

Size: 100uL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 96


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Validation submitted by One World Lab. A431 cells were stained with ARMC3 Polyclonal Antibody, Unconjugated (bs-7969R) at 1:100 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


SMMC7721 cells were stained with ARMC3 Polyclonal Antibody, Unconjugated (bs-7969R) at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ARMC3 Polyclonal Antibody, Unconjugated (bs-7969R) at 1:400 overnight at 4°C, DAB staining.