bs-8027R [Primary Antibody]
CNIH2/3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CNIH2/3

Specificity: This antibody detects both CNIH2 and CNIH3 proteins

Immunogen Range: 25-120/160


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 254263; 149111

Swiss Prot: Q6PI25; Q8TBE1

Source: KLH conjugated synthetic peptide derived from human CNIH2/CNIH3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

CNIH2 and CNIH3 regulate the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). They promote their targeting to the cell membrane and synapses and modulate their gating properties by regulating their rates of activation, deactivation and desensitization. CNIH2 also blocks CACNG8-mediated resensitization of AMPA receptors.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 19


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CNIH3 Polyclonal Antibody, Unconjugated (bs-8027R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Antibody incubation with CNIH3 Polyclonal Antibody, Unconjugated (bs-8027R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.