bs-8177R [Primary Antibody]
KIF11 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: K11

Immunogen Range: 351-450/1056


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3832

Swiss Prot: P52732

Source: KLH conjugated synthetic peptide derived from human KIF11/Eg5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 120


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Junjun Ling. et al. KIF11, a plus end-directed kinesin, as a key gene in benzo(a)pyrene-induced non-small cell lung cancer. Environ Toxicol Phar. 2022 Jan;89:103775Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti KIF11/Eg5/TRIP5 Polyclonal Antibody, Unconjugated (bs-8177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti KIF11/Eg5/TRIP5 Polyclonal Antibody, Unconjugated (bs-8177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 rat thyriod lysates L2 rat brain lysates probed with Anti KIF11/Eg5/TRIP5 Polyclonal Antibody, Unconjugated (bs-8177R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 120kD. Observed band size:120kD.\n


Formalin-fixed and paraffin embedded laryngeal cancer labeled with Rabbit Anti-KIF11 Polyclonal Antibody, Unconjugated (bs-8177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n