bs-8179R [Primary Antibody]
Eg5(Thr926) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Eg5 Thr926

Modification Site: Thr926

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3832

Swiss Prot: P52732

Source: KLH conjugated synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926 [GT(p-T)PQ]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 120


Cross Reactive Species: Human

Predicted Cross Reactive Species: Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human HL60 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human U251 cell lysates probed with Eg5(Thr926) Polyclonal Antibody, Unconjugated (bs-8179R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.