bs-8299R [Primary Antibody]
TXNRD1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TXNRD1

Immunogen Range: 451-550/649


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7296

Swiss Prot: Q16881

Source: KLH conjugated synthetic peptide derived from human TXNRD1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kaya, Bulent, et al. "Intratumoral hemorrhage-related differences in the expression of vascular endothelial growth factor, basic fibroblast growth factor and thioredoxin reductase 1 in human glioblastoma." Molecular and Clinical Oncology 5.4 (2016): 343-346.Read more>>
  • Huafeng Wang. et al. Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. J BIOL CHEM. 2022 May;:102077Read more>>
  • Nedret Kilic. et al. Thioredoxin system and miR-21, miR-23a/b and let-7a as potential biomarkers for brain tumor progression: Preliminary Case Data. WORLD NEUROSURG. 2022 SepRead more>>
  • Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436Read more>>
VALIDATION IMAGES

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 28kD, 55kD.


Formalin-fixed and paraffin embedded human kidney labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat testis labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Brain; 40ug loaded in each lane; Probed with TXNRD1 Polyclonal Antibody, unconjugated (bs-8299R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:400 overnight at 4°C, followed by secondary and and DAB staining.