bs-8299R
[Primary Antibody]
TXNRD1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TXNRD1
Immunogen Range: 451-550/649
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7296
Swiss Prot: Q16881
Source: KLH conjugated synthetic peptide derived from human TXNRD1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:50-200)
IHC-F(IHC-F=1:50-200)
IP(IP=1:20-50)
IF(IF=1:50-200)
Flow-Cyt(Flow-Cyt=1:20-50)
ICC/IF(ICC/IF=1:50-200)
Predicted Molecular Weight: 71
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Kaya, Bulent, et al. "Intratumoral hemorrhage-related differences in the expression of vascular endothelial growth factor, basic fibroblast growth factor and thioredoxin reductase 1 in human glioblastoma." Molecular and Clinical Oncology 5.4 (2016): 343-346.Read more>>
- Huafeng Wang. et al. Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. J BIOL CHEM. 2022 May;:102077Read more>>
- Nedret Kilic. et al. Thioredoxin system and miR-21, miR-23a/b and let-7a as potential biomarkers for brain tumor progression: Preliminary Case Data. WORLD NEUROSURG. 2022 SepRead more>>
- Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human kidney labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TXNRD1 Polyclonal Antibody, Unconjugated (bs-8299R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining
25 ug total protein per lane of various lysates (see on figure) probed with TXNRD1 polyclonal antibody, unconjugated (bs-8299R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with TXNRD1 polyclonal antibody, unconjugated (bs-8299R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.