DATASHEET
Host:
Rabbit
Target Protein:
HDHD3
Immunogen Range:
1-100/251
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
81932
Source:
KLH conjugated synthetic peptide derived from human HDHD3
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
HDHD3 (haloacid dehalogenase-like hydrolase domain containing 3) is a 251 amino acid protein that belongs to the HAD-like hydrolase superfamily, which contains a group of hydrolase enzymes that differ from the å/ hydrolase family based on structure. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD3 is encoded by a gene located on human chromosome 9q32. Chromosome 9 consists of about 145 million bases, 4% of the human genome and encodes nearly 900 genes. Thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype.
VALIDATION IMAGES
Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates probed with HDHD3 Polyclonal Antibody, Unconjugated (bs-8318R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HDHD3) Polyclonal Antibody, Unconjugated (bs-8318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.