bs-8481R [Primary Antibody]
MHC Class 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MHC Class 2

Immunogen Range: 111-200/263


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3109

Swiss Prot: P28068

Source: KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 27


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xueli Bai. et al. Methionine enkephalin activates autophagy and stimulates tumour cell immunogenicity in human cutaneous squamous cell carcinoma. Int Immunopharmacol. 2021 Jul;96:107733Read more>>
  • Pengfei Li. et al. Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages. Front Immunol. 2021; 12: 700009Read more>>
  • Fenghong Wang. et al. Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathway. Free Radical Bio Med. 2022 Mar;182:171Read more>>
  • Yin, Lili. et al. Extracellular vesicles derived from M2-polarized tumor-associated macrophages promote immune escape in ovarian cancer through NEAT1/miR-101-3p/ZEB1/PD-L1 axis. CANCER IMMUNOL IMMUN. 2022 Nov;:1-16Read more>>
  • Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047Read more>>
  • Tao Bi. et al. Zhilong Huoxue Tongyu capsule alleviates myocardial fibrosis by improving endothelial cell dysfunction. Journal of Traditional and Complementary Medicine. 2023 JulRead more>>
  • Peier Chen. et al. Targeted heart repair by T4-loaded cardiac-resident macrophage-derived extracellular vesicles modified with monocyte membranes. ACTA BIOMATER. 2023 AugRead more>>
  • Cunli Yin. et al. LncRNA-HOXC-AS2 regulates tumor-associated macrophage polarization through the STAT1/SOCS1 and STAT1/CIITA pathways to promote the progression of non-small cell lung cancer. CELL SIGNAL. 2024 Mar;115:111031Read more>>
  • Huang Ziyun. et al. Galectin-receptor interaction: a key player in liver fibrosis induced by Schistosoma japonicum infection. PARASITE VECTOR. 2024 Dec;17(1):1-21Read more>>
VALIDATION IMAGES

Mouse lung lysates probed with Anti MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 27kD. Observed band size: 30kD


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-MHC Class II Polyclonal Antibody, Unconjugated (bs-8481R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n