bs-8539R-Biotin

DATASHEET

Host: Rabbit

Target Protein: Luciferase

Immunogen Range: 101-220/550

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7100

Source: KLH conjugated synthetic peptide derived from Firefly Luciferase

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 61

Cross Reactive Species: Tags
Others
(Photinus pyralis (Common eastern firefly) )

For research use only. Not intended for diagnostic or therapeutic use.