bs-8551R
[Primary Antibody]
Nitrotyrosine Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Nitrotyrosine
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated to Nitrotyrosine
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
Others
(Nitrotyrosine)
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Vendrov, Aleksandr, et al. "NOX4 NADPH Oxidase-dependent Mitochondrial Oxidative Stress in Aging-associated Cardiovascular Disease." Antioxidants and Redox Signaling ja (2015).Read more>>
- Chen, Yu-Lin, and Wai-Ming Kan. "Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research (2015).Read more>>
- Wang, Lin-Feng, et al. "Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes." American Journal of Translational Research 7.10 (2015): 1724-1735.Read more>>
- Li, Dawei, et al. "Hepatic Hypoxia-Inducible Factors Inhibit PPARα Expression To Exacerbate Acetaminophen induced Oxidative Stress And Hepatotoxicity." Free Radical Biology and Medicine (2017).Read more>>
- Li et al. Targeting intestinal epithelial cell-programmed necrosis alleviates tissue injury after intestinal ischemia/reperfusion in rats. (2018) J.Surg.Res. 225:108-117Read more>>
- Canonica J et al. Effect of acute and chronic aldosterone exposure on the retinal pigment epithelium-choroid complex in rodents. Exp Eye Res. 2019 Aug 5;187:107747.Read more>>
- Feng Y et al. Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway. Oxid Med Cell Longev. 2019 Nov 6;2019:7067619.Read more>>
- Ahmed S et al. Cocoa Flavonoids Reduce Inflammation and Oxidative Stress in a Myocardial Ischemia-Reperfusion Experimental Model. Antioxidants (Basel). 2020 Feb 18;9(2). pii: E167.Read more>>
- Yan Xuet al. Blockade of Platelets Using Tumor-Specific NO-Releasing Nanoparticles Prevents Tumor Metastasis and Reverses Tumor Immunosuppression. ACS Nano. 2020 Aug 25;14(8):9780-9795.Read more>>
- Chuang Yang. et al. PPAR down-regulation is involved in high glucose-induced endothelial injury via acceleration of nitrative stress. Microvasc Res. 2022 Jan;139:104272Read more>>
- Yuan Chen. et al. Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis. Acs Nano. 2022;XXXX(XXX):XXX-XXXRead more>>
- Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXXRead more>>
- Chuang Yang. et al. Gastrodin protects endothelial cells against high glucose-induced injury through up-regulation of PPAR and alleviation of nitrative stress. MICROVASC RES. 2023 Jul;148:104531Read more>>
- Aleyna ?ztzn. et al. Inflammatory signal transduction pathways induced by prilocaine toxicity in cultured ARPE-19 cells. J BIOCHEM MOL TOXIC. 2023 Aug;:e23491Read more>>
- Siyuan Wang. et al. Inhibition of cisplatin-induced Acsl4-mediated ferroptosis alleviated ovarian injury. CHEM-BIOL INTERACT. 2024 Jan;387:110825Read more>>
VALIDATION IMAGES
Hela probed with Nitrotyrosine Polyclonal Antibody, Unconjugated (bs-8551R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Cpnjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Nitrotyrosine Polyclonal Antibody, Unconjugated (bs-8551R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.