bs-8555R [Primary Antibody]
Desmuslin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Desmuslin

Immunogen Range: 501-650/1565


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23336

Swiss Prot: O15061

Source: KLH conjugated synthetic peptide derived from human Desmuslin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 172


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Bengoechea, Rocio, et al. "Myofibrillar disruption and RNA binding protein aggregation in a mouse model of limb girdle muscular dystrophy 1D." Human Molecular Genetics (2015): ddv363.Read more>>
  • Bengoechea et al. Myofibrillar disruption and RNA-binding protein aggregation in a mouse model of limb-girdle muscular dystrophy 1D. (2015) Hum.Mol.Genet. 24:6588-602Read more>>
  • Jipeng Jiang. et al. 3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine. Biomater Sci-Uk. 2020 Nov;8(22):6362-6374Read more>>
  • Weihl, Conrad C.. et al. Loss of function variants in DNAJB4 cause a myopathy with early respiratory failure. ACTA NEUROPATHOL. 2022 Oct;:1-17Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Desmuslin Polyclonal Antibody, Unconjugated (bs-8555R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes.