bs-8557R [Primary Antibody]
DSPP Polyclonal Antibody
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Host: Rabbit

Target Protein: DSPP

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1834

Source: KLH conjugated synthetic peptide derived from human DSPP/Dentin phosphophoryn

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

DSPP may be an important factor in dentinogenesis. DSPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.The dentin sialophosphoprotein (DSPP) gene encodes two major noncollagenous dentin matrix proteins: dentin sialoprotein, known as DSP; and dentin phosphoprotein, known as phosphophoryn or DPP. The DSPP gene maps to human chromosome 4 and yields a 4.6 kb transcript containing 5 exons, with exons 1-4 encoding for DSP and exon 5 encoding for both the C-terminus of DSP and the full length DPP. DPP and DSP are acidic proteins that represent about 50% of the noncollagenous proteins in the dentin extracellular matrix of teeth. The DSPP gene is a likely candidate gene for the genetic diseases associated with abnormal dentin formation such as dentinogenesis imperfecta type II and dentin dysplasia type II. DPP may bind large amounts of calcium and may facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun N et al. Graphene oxide-coated porous titanium for pulp sealing: an antibacterial and dentino-inductive restorative material. J Mater Chem B. 2020 Jul 8;8(26):5606-5619.Read more>>
  • Yongtao Li. et al. Effects of epigallocatechin gallate (EGCG) on the biological properties of human dental pulp stem cells and inflammatory pulp tissue. Arch Oral Biol. 2021 Mar;123:105034Read more>>
  • Liu, Yangqiu. et al. Functional expression of TRPA1 channel, TRPV1 channel and TMEM100 in human odontoblasts. J Mol Histol. 2021 Oct;52(5):1105-1114Read more>>
  • Natsuki Saito. et al. Gαs-Coupled CGRP Receptor Signaling Axis from the Trigeminal Ganglion Neuron to Odontoblast Negatively Regulates Dentin Mineralization. BIOMOLECULES. 2022 Dec;12(12):1747Read more>>
  • Sadao Ohyama. et al. Piezo1-pannexin-1-P2X3 axis in odontoblasts and neurons mediates sensory transduction in dentinal sensitivity. FRONT PHYSIOL. 2022; 13: 891759Read more>>
  • Yuanyuan Shi. et al. Decellularized rat submandibular gland as an alternative scaffold for dental pulp regeneration. FRONT BIOENG BIOTECH. 2023; 11: 1148532Read more>>
  • Eri Kitayama. et al. Functional Expression of IP, 5-HT4, D1, A2A, and VIP Receptors in Human Odontoblast Cell Line. BIOMOLECULES. 2023 Jun;13(6):879Read more>>
  • Zhang MengDan. et al. Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathway. BIOL DIRECT. 2023 Dec;18(1):1-16Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded bone of mouse embryo labeled with Anti-DSPP Polyclonal Antibody, Unconjugated (bs-8557R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human Hela cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with DSPP Polyclonal Antibody, Unconjugated (bs-8557R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.