bs-8571R-A555

DATASHEET

Host: Rabbit

Target Protein: Histone H2A.X Ubiquityl Lys119

Specificity: This modification site is homologous to that of Lys119 in Rat.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3014

Swiss Prot: P16104

Source: KLH conjugated synthetic peptide derived from human Ubiquityl Histone H2A.X (Lys119)

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Conjugation: ALEXA FLUOR® 555

Excitation/ Emission: 555nm/580nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.