bs-8585R [Primary Antibody]
Arginase 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Arginase 1

Immunogen Range: 151-220/322


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 383

Swiss Prot: P05089

Source: KLH conjugated synthetic peptide derived from human Arginase 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Arginase I which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea . The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100)
ICC(1:100)

Predicted Molecular Weight: 35


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS nano (2015).Read more>>
  • Huang, Ling, et al. "Enhancement of Local Bone Remodeling in Osteoporotic Rabbits by Biomimic Multilayered Structures on Ti6Al4V Implants." Journal of Biomedical Materials Research Part A (2016).Read more>>
  • EMŐKE HORVÁTH et al. FISH-OIL PRECONDITIONING UP-REGULATES EXPRESSION OF SPLENIC ARG1 POSITIVE M2 TYPE MACROPHAGES AND THE ARG1/INOS2 RATIO AFTER EXPERIMENTAL INDUCED TRANSIENT CEREBRAL ISCHEMIA. FARMACIA, 2019, Vol. 67, 5.Read more>>
  • Sun Y et al. Apple polysaccharide prevents from colitis-associated carcinogenesis through regulating macrophage polarization. Int J Biol Macromol. 2020 Oct 15;161:704-711.Read more>>
  • Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.Read more>>
  • Peng Yuan. et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology. 2020;14(8):1137-1155Read more>>
  • Lushuang Xie. et al. Inflammatory factors and amyloid -induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538C22549Read more>>
  • Gaoyang Guo. et al. A method for simultaneously crosslinking and functionalizing extracellular matrix-based biomaterials as bioprosthetic heart valves with enhanced endothelialization and reduced inflammation. Acta Biomater. 2021 Jan;119:89Read more>>
  • Guanglin Zhang. et al. Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects. Acta Biomater. 2021 JulRead more>>
  • Xueyang Zhao. et al. Improved tribological properties, cyto-biocompatibility and anti-inflammatory ability of additive manufactured Ti-6Al-4V alloy through surface texturing and nitriding. Surf Coat Tech. 2021 Nov;425:127686Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-Arginase 1 Polyclonal Antibody, Unconjugated (bs-8585R) at 1:200 followed by conjugation to the secondary antibody and DAB staining