bs-8680R-A488 [Conjugated Primary Antibody]
IRE1a Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRE1a

Immunogen Range: 252-260/977


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2081

Swiss Prot: O75460

Source: KLH conjugated synthetic peptide derived from human IRE1a

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 490nm/525nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 105


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiang, H-Q., et al. "Guanabenz Delays the Onset of Disease Symptoms, Extends Lifespan, Improves Motor Performance and Attenuates Motor Neuron Loss in the SOD1 G93A Mouse Model of Amyotrophic Lateral Sclerosis." Neuroscience (2014).Read more>>
  • Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015).Read more>>
  • Li, Xiaoyan, Fang Han, and Yuxiu Shi. "IRE1α-XBP1 Pathway Is Activated Upon Induction of Single-Prolonged Stress in Rat Neurons of the Medial Prefrontal Cortex." Journal of Molecular Neuroscience (2015): 1-10.Read more>>
  • Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226.Read more>>
  • Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176.Read more>>
  • Chen, Li, et al. "Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid." Endocrine (2016): 1-13.Read more>>
  • He, Wencheng, et al. "CSFV Infection Up-Regulates the Unfolded Protein Response to Promote Its Replication." Frontiers in Microbiology 8 (2017): 2129.Read more>>
  • Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.Read more>>
  • Kishi,et al.Pro-metastatic signaling of the trans fatty acid elaidic acid is associated with lipid rafts.(2018) Oncology Letters. 15:4423-4426.Read more>>
  • Du,et al.A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP.(2018) BioMed Research International. 2018:1761865.Read more>>
  • Lu, Y. et al. Protective effects of Danzhi jiangtang capsule on vascular endothelial damages induced by high-fat diet and palmitic acid. (2018) Biomedicine & Pharmacotherapy, 107, 1631–1640. Read more>>
  • Cui J et al. Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress-and Ca2. Oxid Med Cell Longev. 2019 Jan 9;2019:2593742. Read more>>
  • Yang S et al. Induction of the unfolded protein response (UPR) during pseudorabies virus infection. Vet Microbiol. 2019 Dec;239:108485.Read more>>
  • Jiajia Chenet al. Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. Neurochem Res. 2020 Oct;45(10):2302-2311.Read more>>
  • Yu-Dong Wei. et al. Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells. Zool Res. 2021 Jan 18; 42(1): 14C27Read more>>
  • Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimers disease via the relief of endoplasmic reticulum stress in A-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16Read more>>
  • Zhang Hao. et al. Protective effects of pterostilbene against hepatic damage, redox imbalance, mitochondrial dysfunction, and endoplasmic reticulum stress in weanling piglets. J Anim Sci. 2020 Oct;98(10)Read more>>
  • Mei Zhang. et al. Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Neurotoxicology. 2022 Jan;88:187Read more>>
  • Gurur Garip. et al. Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death. ACTA HISTOCHEM. 2022 Oct;124:151951Read more>>
  • Zhu, Zhenglin. et al. The natural product salicin alleviates osteoarthritis progression by binding to IRE1 and inhibiting endoplasmic reticulum stress through the IRE1-IB-p65 signaling pathway. EXP MOL MED. 2022 Nov;:1-13Read more>>
  • Angelica D. Reyes-Perea. et al. The Mycotoxin De-Epoxy-Deoxynivalenol (DOM-1) Increases Endoplasmic Reticulum Stress in Ovarian Theca Cells. TOXINS. 2023 Mar;15(3):228Read more>>
VALIDATION IMAGES