bs-8687R
[Primary Antibody]
p53 (FL-393) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: p53 FL-393
Immunogen Range: 1-393/393
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7157
Swiss Prot: P04637
Source: Full length human p53 protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 53
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Qin, Qin, et al. "Effects of irreversible electroporation on cervical cancer cell lines in vitro." Molecular Medicine Reports 14.3 (2016): 2187-2193.Read more>>
- Zeng, Kun, et al. "Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice." Bioscience Reports (2017): BSR20170695.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Zhan C et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2019 Oct 1. pii: S0378-4274(19)30295-4. Read more>>
- Sola M et al. Tau affects P53 function and cell fate during the DNA damage response. Commun Biol. 2020 May 19;3(1):245.Read more>>
- Jacquelyn Powers. et al. A Rare TP53 Mutation Predominant in Ashkenazi Jews Confers Risk of Multiple Cancers. Cancer Res. 2020 Sep;80(17):3732-3744Read more>>
- McCann, Jennifer J.. et al. Mutant p53 elicits context-dependent pro-tumorigenic phenotypes. Oncogene. 2021 Nov;:1-15Read more>>
- Hao, Yanan. et al. Gut microbiota-testis axis: FMT improves systemic and testicular micro-environment to increase semen quality in type 1 diabetes. MOL MED. 2022 Dec;28(1):1-17Read more>>
- Merle Nastasja. et al. Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase. MOL CANCER. 2022 Dec;21(1):1-22Read more>>
- Shakur Mohibi. et al. ZFP14 Regulates Cancer Cell Growth and Migration by Modulating p53 Protein Stability as Part of the MDM2 E3 Ubiquitin Ligase Complex. CANCERS. 2022 Jan;14(21):5226Read more>>
- Hanhan Fang. et al. Gut-Spleen Axis: Microbiota via Vascular and Immune Pathways Improve Busulfan-Induced Spleen Disruption | mSphere. MSPHERE. 2022 DecRead more>>
- Liu, Zhenni. et al. CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence. INT J BIOL SCI. 2023 Jan;19(3):950-966Read more>>
- Yanxia Wu. et al. SCARNA10 regulates p53 acetylation-dependent transcriptional activity. BIOCHEM BIOPH RES CO. 2023 Aug;669:38Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat bladder tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Human 293T Cell lysate (30ug), probed with p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:300 overnight at 4˚C. Followed by incubation with secondary antibody at 1:20000 for 90min at 37C.
Human MCF-7 Cell lysate (40ug), probed with p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:300 overnight at 4˚C. Followed by incubation with secondary antibody at 1:20000 for 90min at 37C.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p53 (FL-393) Polyclonal Antibody, Unconjugated (bs-8687R) at 1:200 overnight at 4°C, DAB staining.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with p53 (FL-393) Polyclonal Antibody(bs-8687R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (p53 (FL-393)) polyclonal Antibody, Unconjugated (bs-8687R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.