bs-8709R [Primary Antibody]
phospho-IRS1 (Tyr1179) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-IRS1 (Tyr1179)

Modification Site: Tyr1179

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3667

Swiss Prot: P35568

Source: KLH conjugated synthesised phosphopeptide derived from human IRS1 around the phosphorylation site of Ser1179.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF()

Cross Reactive Species: Human
Mouse
Zebrafish

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Chicken
Rabbit
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS1 (Tyr1179) Polyclonal Antibody(bs-8709R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).