bs-8747R [Primary Antibody]
Phospholipase D2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospholipase D2

Immunogen Range: 21-120/933


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5338

Swiss Prot: O14939

Source: KLH conjugated synthetic peptide derived from human PLD2/Phospholipase D2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May have a role in signal-induced cytoskeletal regulation and/or endocytosis.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 106


Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Sheep
Rabbit
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospholipase D2 Polyclonal Antibody, Unconjugated (bs-8747R) at 1:200 overnight at 4°C, DAB staining.