bs-8987R [Primary Antibody]
Connexin 43 Polyclonal Antibody
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Host: Rabbit

Target Protein: Connexin 43

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2697

Source: KLH conjugated synthetic peptide derived from human Connexin 43

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq].

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:20-100)
IHC-F(1:200-400)
IF(1:50-200)
Flow-Cyt(1ug/Test)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ying Cao. et al. Effects of different dosages esketamine on cardiac conduction and heterogeneity of Cx43: the epicardial mapping in guinea pigs. ANN TRANSL MED. 2022 Jul; 10(14): 772Read more>>
  • Huang Jing. et al. Effects of exposure to PM2.5 during pregnancy on the multigenerational reproductive outcomes of male mouse offspring and the role of Sertoli cells. ENVIRON SCI POLLUT R. 2023 Sep;:1-13Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Connexin 43 Polyclonal Antibody, Unconjugated (bs-8987R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Mouse Stomach tissue lysates probed with Connexin 43 Polyclonal Antibody, Unconjugated (bs-8987R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.