Host: Rabbit
Target Protein: CD34
Immunogen Range: 181-280/385
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 947
Swiss Prot: P28906
Source: KLH conjugated synthetic peptide derived from human CD34
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Mouse
Rat
Pig
For research use only. Not intended for diagnostic or therapeutic use.
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Mouse heart lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\n
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining
Lane 1: Mouse Ovary; Probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Lung; Probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.