Host: Rabbit
Target Protein: RGC32
Immunogen Range: 65-137/137
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 28984
Swiss Prot: Q9H4X1
Source: KLH conjugated synthetic peptide derived from human RGC32
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 15
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Tatomir A et al. RGC-32 regulates reactive astrocytosis and extracellular matrix deposition in experimental autoimmune encephalomyelitis.Immunol Res. 2018 Jul 13.Read more>>
- Tatomir A et al. RGC-32 regulates reactive astrocytosis and extracellular matrix deposition in experimental autoimmune encephalomyelitis.Immunol Res. 2018 Jul 13.Read more>>
- Sonia I. Vlaicu. et al. RGC-32_ dual role in smooth muscle cells and atherogenesis. CLIN IMMUNOL. 2022 May;238:10902Read more>>
- Nian-jie Zhang. et al. Nonylphenol Promoted EpithelialCMesenchymal Transition in Colorectal Cancer Cells by Upregulating the Expression of Regulator of Cell Cycle. CHEM RES TOXICOL. 2022;35(9):1533C154Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-RGC32 Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
This image was kindly submitted by Horea Rus of the University of Maryland.\nParaffin embedded Human kidney tissue; Stained with Rabbit Anti-RGC-32 Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 dilution followed by the RTU Vectastain kit from Vector.
This image was kindly submitted by Horea Rus of the University of Maryland.\nParaffin embedded Human kidney tissue; Stained with Rabbit Anti-RGC-32 Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 dilution followed by the RTU Vectastain kit from Vector.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-9079R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.