bs-9092R [Primary Antibody]
hnRNP U Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: hnRNP U

Immunogen Range: 2-100/825


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3192

Swiss Prot: Q00839

Source: KLH conjugated synthetic peptide derived from human hnRNP U

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. However, its role in the regulation of gene expression is as yet poorly understood. Proteins of the heterogeneous nuclear ribonucleoparticles (hnRNP) family form a structurally diverse group of RNA binding proteins implicated in various functions. Recently, hnRNP proteins have been shown to hinder communication between factors bound to different splice sites. Conversely, several reports have described a positive role for some hnRNP proteins in pre-mRNA splicing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:50-200)
ICC/IF(ICC/IF=1:50)

Cross Reactive Species: Human
Mouse
Rat
Others

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1:Mouse cerebrum lysates; Lane 2: Rat cerebrum lysates probed with hnRNP U Polyclonal Antibody, Unconjugated (bs-9092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human HepG2 cell lysates probed with hnRNP U Polyclonal Antibody, Unconjugated (bs-9092R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.


Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (hnRNP U) Polyclonal Antibody, Unconjugated (bs-9092R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.