Host: Rabbit
Target Protein: RNF138
Immunogen Range: 51-150/245
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 51444
Swiss Prot: Q8WVD3
Source: KLH conjugated synthetic peptide derived from human RNF138
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 28
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:400 overnight at 4°C, DAB staining.