DATASHEET
Host:
Rabbit
Target Protein:
RNF170
Immunogen Range:
101-200/258
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
81790
Source:
KLH conjugated synthetic peptide derived from human RNF170
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The ring finger is a specialized type of zinc finger of 40 to 60 residues that binds two atoms of zinc and mediates protein-protein interactions. There are five known isoforms of RNF170.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4°C, followed by SP Kit (Rabbit) and DAB.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RNF170 Polyclonal Antibody (bs-9260R) at 1:400 overnight at 4°C, followed by SP Kit (Rabbit) and DAB.