bs-9308R [Primary Antibody]
KATNA1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: KATNA1

Immunogen Range: 221-320/491


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11104

Source: KLH conjugated synthetic peptide derived from human KATNA1/Katanin p60 A1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Microtubules are polymers of alpha and beta subunits that form the mitotic spindle and assist in the organization of membranous organelles during interphase. Katanin p60 A1, also known as KATNA1, is a 491 amino acid protein that belongs to the AAA ATPase family and is involved in microtubule regulation. Localized to the cytoplasm and to the centrosome, Katanin p60 A1 functions to sever and disassemble microtubules in an ATP-dependent manner, thus promoting the rapid reorganization of cellular microtubule arrays and playing an important role in microtubule release from the centrosome after nucleation. Katanin p60 A1, which exists as two alternatively spliced isoforms, can homooligomerize into hexameric rings whose activity is stimulated by the presence of microtubules.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human Jurkat cell lysates probed with KATNA1 Polyclonal Antibody, Unconjugated (bs-9308R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (KATNA1) Polyclonal Antibody, Unconjugated (bs-9308R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.