DATASHEET
Host:
Rabbit
Target Protein:
NNF1R
Immunogen Range:
101-200/300
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
11243
Source:
KLH conjugated synthetic peptide derived from human NNF1R/PMF1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
NNF1R, also called PMF1, is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. It is required for chromosome congression and for correct operation of the spindle checkpoint. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
VALIDATION IMAGES
Lane 1: MDA-MB-231 cell lysates probed with NNF1R Polyclonal Antibody, Unconjugated (bs-9319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NNF1R Polyclonal Antibody, Unconjugated (bs-9319R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NNF1R Polyclonal Antibody, Unconjugated (bs-9319R) at 1:200 overnight at 4°C, DAB staining.