bs-9341R [Primary Antibody]
MARCH7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: 7-Mar

Immunogen Range: 31-130/704


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 64844

Swiss Prot: Q9H992

Source: KLH conjugated synthetic peptide derived from human MARCH7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IP(1-2ug)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hu, Jianguo, et al. "Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth." Oncotarget 6.14 (2015): 12174.Read more>>
  • Zhang, Li, et al. "Expression of USP7 and MARCH7 Is Correlated with Poor Prognosis in Epithelial Ovarian Cancer." The Tohoku Journal of Experimental Medicine 239.3 (2016): 165-175.Read more>>
  • Hu et al. Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway. (2018) Oncol.Lett. 16:2312-2318Read more>>
  • Liu L et al. miR-27b-3p/MARCH7 regulates invasion and metastasis of endometrial cancer cells through Snail-mediated pathway. Acta Biochim Biophys Sin (Shanghai). 2019 Apr 22. pii: gmz030. Read more>>
  • Xu M et al. Acetate attenuates inflammasome activation through GPR43-mediated Ca 2+-dependent NLRP3 ubiquitination. Exp Mol Med. 2019 Jul 23;51(7):83. Read more>>
VALIDATION IMAGES

MCF-7 Cell lysates probed with MARCH7 Polyclonal Antibody, unconjugated (bs-9341R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.