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Host: Rabbit
Target Protein: ANKRD33B
Immunogen Range: 401-494/494
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 651746
Swiss Prot: A6NCL7
Source: KLH conjugated synthetic peptide derived from human ANKRD33B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100µL
Concentration: 1ug/ul
Applications:
WB(1:500-2000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
Predicted Molecular Weight: 54
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: mouse erebellum lysates; Lane 2: mouse hippocampus lysates probed with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat cerebral cortex lysates probed with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4°C, DAB staining.