bs-9468R [Primary Antibody]
Nebulette Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nebulette

Immunogen Range: 321-420/1014


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10529

Swiss Prot: O76041

Source: KLH conjugated synthetic peptide derived from human Nebulette

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 116


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ying He. et al. Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma. MED SCI MONITOR. 2022; 28: e937081-1e937081-17Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-Nebulette Polyclonal Antibody, Unconjugated (bs-9468R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Pancreas lysates probed with Nebulette Polyclonal Antibody, Unconjugated (bs-9468R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.