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Host: Rabbit
Target Protein: PAR4
Immunogen Range: 214-247/385
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9002
Swiss Prot: Q96RI0
Source: KLH conjugated synthetic peptide derived from human PAR4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100uL
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Mouse spleen lysate probed with Rabbit Anti-PAR4 Polyclonal Antibody (bs-9511R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT
Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Lung tissue lysates; Lane 4: Rat Thyroid gland tissue lysates ; Lane 5: Rat Testis tissue lysates ; Lane 6: Human K562 cell lysates ; Lane 7: Human THP-1 cell lysates ; Lane 8: Human Hela cell lysates ; Lane 9: Human Huvec cell lysates probed with Proteinase-activated receptor 4/PAR4 Polyclonal Antibody, Unconjugated (bs-9511R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.