bs-9877R [Primary Antibody]
Staufen Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Staufen

Immunogen Range: 421-520/577


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6780

Swiss Prot: O95793

Source: KLH conjugated synthetic peptide derived from human Staufen

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 63


Cross Reactive Species: Human
Mouse
Rat
Chicken
Horse
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • de Morrée, Antoine, et al. "Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence." Proceedings of the National Academy of Sciences 114.43 (2017): E8996-E9005.Read more>>
  • Morree et al. Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence. (2017) Proc.Natl.Acad.Sci.U.S.A. 114:E8996-E9005Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


SW480 cell lysates probed with Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.