bs-9878R [Primary Antibody]
Cyclophilin 40 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cyclophilin 40

Immunogen Range: 281-370/370


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5481

Source: KLH conjugated synthetic peptide derived from human Cyclophilin 40

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved from yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It is a widely expressed cytoplasmic protein.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human 293T cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human K562 cell lysates; Lane 8: Human Jurkat cell lysates probed with Cyclophilin 40/PPID Polyclonal Antibody, Unconjugated (bs-9878R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.