bsm-0287M [Primary Antibody]
His tag (2D5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: His tag

Clonality: Monoclonal

Isotype: IgG

Source: KLH conjugated HHHHHH peptide

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Tags
Others
(HHHHHH tag)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Yun, et al. "Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection." Infection and immunity 80.8 (2012): 2948-2955.Read more>>
  • Li, Mo-Fei, et al. "A peptidoglycan recognition protein from< i> Sciaenops ocellatus</i> is a zinc amidase and a bactericide with a substrate range limited to Gram-positive bacteria." Fish & Shellfish Immunology (2011).Read more>>
  • Yu, Lan-ping, et al. "Characterization of a c-type lysozyme of Scophthalmus maximus: expression, activity, and antibacterial effect." Fish & Shellfish Immunology (2012).Read more>>
  • Zhang, Bao-cun, et al. "Rock bream (< i> Oplegnathus fasciatus</i>) viperin is a virus-responsive protein that modulates innate immunity and promotes resistance against megalocytivirus infection." Developmental & Comparative Immunology (2014).Read more>>
  • Qin, Jialin, et al. "Selective and sensitive homogenous assay of serum albumin with 1-anilinonaphthalene-8-sulphonate as a biosensor." Analytica Chimica Acta (2014).Read more>>
  • Zhou, Ze-jun, and Li Sun. "CsCTL1, a teleost c-type lectin that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved EPN motif." Developmental & Comparative Immunology (2015).Read more>>
  • Wang, Yan, et al. "Enhanced tolerance and remediation to mixed contaminates of PCBs and 2, 4-DCP by transgenic alfalfa plants expressing the 2, 3-dihydroxybiphenyl-1, 2-dioxygenase." Journal of Hazardous Materials (2014).Read more>>
  • Zhang J, Zhang Bc, Sun L (2015) P247 and P523: Two In Vivo-Expressed Megalocytivirus Proteins That Induce Protective Immunity and Are Essential to Viral Infection. PLoS ONE 10(3): e0121282Read more>>
  • Wang, S. M., et al. "A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting." Clinical, Cosmetic and Investigational Dermatology 8 (2015): 193-204.Read more>>
  • Zhang, Bao-cun, and Li Sun. "Tongue sole (Cynoglossus semilaevis) prothymosin alpha: cytokine-like activities associated with the intact protein and the C-terminal region that lead to antiviral immunity via Myd88-dependent and–independent pathways respectively." Developmental & Comparative Immunology (2015).Read more>>
  • Sun, Qing-lei, and Li Sun. "A short-type peptidoglycan recognition protein from tongue sole (Cynoglossus semilaevis) promotes phagocytosis and defense against bacterial infection." Fish & Shellfish Immunology (2015).Read more>>
  • Sun, Yuan-yuan, Li Liu, and Jun Li. "Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria." Developmental & Comparative Immunology (2015).Read more>>
  • Li, Mo-fei, and Jian Zhang. "CsTNF1, a teleost tumor necrosis factor that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved receptor binding site." Developmental & Comparative Immunology (2015).Read more>>
  • Li, Mo-fei, and Yong-hua Hu. "C5a of Cynoglossus semilaevis has anaphylatoxin-like properties and promotes antibacterial and antiviral defense." Developmental & Comparative Immunology (2016).Read more>>
  • Gao, Lili, et al. "The role of a novel C-type lectin-like protein from planarian in innate immunity and regeneration." Developmental & Comparative Immunology (2016).Read more>>
  • Li, Mo-fei, Jun Li, and Li Sun. "CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity." Scientific Reports 6 (2016): 39287.Read more>>
  • Kumar V et al. PirABVP Toxin Binds to Epithelial Cells of the Digestive Tract and Produce Pathognomonic AHPND Lesions in Germ-Free Brine Shrimp. Toxins (Basel). 2019 Dec 9;11(12). pii: E717.Read more>>
  • Yu Zhang. et al. An adeno-associated virus-mediated immunotherapy for Alzheimers disease. Mol Immunol. 2022 Apr;144:26Read more>>
VALIDATION IMAGES

Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug Primary: Anti-His tag (bsm-0287M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD