Host: Mouse
Target Protein: Human IgM
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3507
Swiss Prot: P01871
Source: Native Human IgM
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human IgM) Monoclonal Antibody, Unconjugated (bsm-0345M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Lane 1: Human Raji cell lysates; Lane 2: Human RAMOS cell lysates; Lane 3: Human Serum cell lysates probed with Human IgM Monoclonal Antibody, Unconjugated (bsm-0345M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.