bsm-0917M [Primary Antibody]
BrdU (A7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Mouse

Target Protein: BrdU

Clonality: Monoclonal

Isotype: IgG

Source: BrdU conjugated to KLH

Purification: Purified by Protein G.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is incorporated into DNA during S-phase of a cell cycle. It is commonly used in the detection of proliferating cells in living tissues

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Others
(BrdU)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Hongwei, et al. "Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress." Brain Research (2013).Read more>>
  • Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.Read more>>
  • Wang, N., et al. "Sodium hyaluronate-CNTF gelatinous particles promote axonal growth, neurogenesis and functional recovery after spinal cord injury." Spinal Cord (2014).Read more>>
  • Zhao, Lan, et al. "Acupuncture Improves Cerebral Microenvironment in Mice with Alzheimer’s Disease Treated with Hippocampal Neural Stem Cells."Molecular Neurobiology (2016): 1-11.Read more>>
  • "Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells.(2018) J. Cell. Biochem. 2018 Oct 18 "Read more>>
  • Lusine Danielyan. et al. Cell motility and migration as determinants of stem cell efficacy. Ebiomedicine. 2020 Oct;60:102989Read more>>
VALIDATION IMAGES

MCF-7 cells were labeled with BrdU (10μM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 μm.


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37°C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37°C


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining